Neurospheres: a potential in vitro model for the study of central nervous system disorders

Neurogenesis was believed to end after the period of embryonic development. However, possibility obtaining an expressive number cells with functional neuronal characteristics implied a great advance in experimental research. New techniques have emerged demonstrate that birth new neurons continues occur adult brain. Two main rich sources these are subventricular zone (SVZ) and subgranular hippocampal dentate gyrus (SGZ) where neural stem (aNSCs) ability proliferate differentiate into mature cell lines. The cultivation neurospheres is method isolate, maintain expand (NSCs) has been used extensively by several research groups analyze biological properties NSCs their potential use injured brains from animal models. Throughout this review, we highlight areas type culture applied advantages limitations using model studies for neurological clinical scenario.